So. I’m installed in the lab. I’ve done my initial share of mixing up buffers and LB media for the E.coli bacteria (the ever-present lab workhorse). I’ve created a (tiny) stock of vectors (pRSET based), and should work out the primers for PCR amplifying the two chitin binding proteins I will work on. Trying not to drown in protocols and all kind of information these first weeks, I’ve set up a wiki, and I think it will be quite useful. Actually, I think the whole lab should switch to the OpenWetWare system. My supervisor seems to like the idea, at least. But I guess the management is sick and tired of this happening every year, with new students trying to change the lab… (I know I would!) Today, I spent most of the day playing around with PyMOL, which provides “real-time visualization and rapid generation of high-quality molecular graphics images and animations“. It will definitely help trying to understand the chitin binding processes. Oh, and by the way: PubCrawler. “It goes to the library – you go to the pub(TM)”. You gotta love it.
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We would be happy to help you setup an example lab page to see what openwetware may be useful for. Feel free to email us (admin at openwetware dot org) if you’d like to talk.
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